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Thermo Scientific™ HIF-1 alpha ELISA Kit, Human


Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Manufacturer: thermo scientific™  EHIF1A

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Catalog No. ENEHIF1A

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Description & Specifications

Specifications

Cross Reactivity Mouse and rat HIF-1A
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Description HIF-1 Alpha ELISA Kit, 96 Assays, 1 Kit, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Reagents
Sensitivity ≤30pg/mL
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Regulatory Status For research use only. Not for use in diagnostic procedures
Assay Range 81.92 to 20,000pg/mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Packaging Quantity 1 Kit(s)
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Assays 96
Quantity 1 Kit(s)
Quantity 96 assays
Formulation Solid
Sufficient For 96 Determinations
Precision Intra-assay CV <10%; inter-assay CV <10%
Research Discipline Immunology
Host Species Human
Species Human
Storage Requirements 2 to 8°C
Sample Size 50μL
Conjugate Streptavidin-HRP
Substrate Type TMB
Size 96 Assay(s)
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Test Time 3.5 hr.
Product Type HIF-1 Alpha ELISA Kit, Human
Total Assay Time 3.5 hr.
For Use With (Application) Protein assays and analysis
Measuring Range 450 to 550nm
Protein Family Transcription Factors
Sample Volume 50μL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Detection Method Colorimetric