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Thermo Scientific™ HIF-1 alpha ELISA Kit, Human


Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Manufacturer: thermo scientific™  EHIF1A5

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Catalog No. ENEHIF1A5

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Description & Specifications

Specifications

Includes Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution.
Quantity 480 assays
Species Human
Storage Requirements Store kit at 2°-8°C
Protein Family Transcription Factors
Research Discipline Immunology
Detection Method Colorimetric

The Thermo Scientific HIF-1 alpha ELISA Kit contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate. This product contains reagents for analyte detection in 96 microwells.

Features of Thermo Scientific HIF-1 alpha ELISA Kits:

  • Target: natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 81.92 to 20,000 pg/mL
  • Sensitivity: < 30 pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture lysate, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50µL
  • Specificity: specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A.

These HIF-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of hypoxia-inducible factor 1-alpha (HIF1A, also called BHLHE78, MOP1, PASD8) in samples at 50 to 100µL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. .


For Research Use Only. Not for use in diagnostic procedures.