Please login to your online account to display your discounted pricing

Thermo Scientific™ HIF-1 alpha ELISA Kit, Human


Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Manufacturer: thermo scientific™  EHIF1A5

 View more versions of this product

Catalog No. ENEHIF1A5

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Protein assays and analysis
Research Discipline Immunology
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Formulation Solid
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Assay Range 81.92 to 20,000pg/mL
Product Type HIF-1 Alpha ELISA Kit, Human
Packaging Quantity 1 Kit(s)
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 Kit(s)
Quantity 480 assays
Regulatory Status For research use only. Not for use in diagnostic procedures
Sensitivity ≤30pg/mL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50μL
Sample Volume 50μL
Size 480 Assay(s)
Species Human
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Storage Requirements 2 to 8°C
Substrate Type TMB
Sufficient For 480 Determinations
Test Time 3.5 hr.
Total Assay Time 3.5 hr.
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Assays 480
Measuring Range 450 to 550nm
Host Species Human
Protein Family Transcription Factors
Conjugate Streptavidin-HRP
Cross Reactivity Mouse and rat HIF-1A
Description HIF-1 Alpha ELISA Kit, 480 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Detection Method Colorimetric