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Thermo Scientific™ HIF-1 alpha ELISA Kit, Human


Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Manufacturer: thermo scientific™  EHIF1A5

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Catalog No. ENEHIF1A5

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Description & Specifications

Specifications

Research Discipline Immunology
For Use With (Application) Protein assays and analysis
Formulation Solid
Measurement Absorbance at 450nm minus absorbance at 550nm
Protein Family Transcription Factors
Detection Method Colorimetric
Quantity 1 Kit(s)
Quantity 480 assays
Description HIF-1 Alpha ELISA Kit, 480 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Assay Range 81.92 to 20,000pg/mL
Measuring Range 450 to 550nm
Conjugate Streptavidin-HRP
Test Time 3.5 hr.
Product Type HIF-1 Alpha ELISA Kit, Human
Sensitivity ≤30pg/mL
Sample Volume 50μL
Sufficient For 480 Determinations
Storage Requirements 2 to 8°C
Sample Size 50μL
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Precision Intra-assay CV <10%; inter-assay CV <10%
Regulatory Status For research use only. Not for use in diagnostic procedures
Total Assay Time 3.5 hr.
Size 480 Assay(s)
Species Human
Substrate Type TMB
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Host Species Human
Assays 480
Cross Reactivity Mouse and rat HIF-1A
Packaging Quantity 1 Kit(s)