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Invitrogen™ HIF-1 alpha ELISA Kit, Human DFS Item

Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Manufacturer:  Invitrogen™ LSEHIF1A2

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Catalog No. ENEHIF1A2



Specifications

Specifications

Colorimetric
Streptavidin-HRP
HIF-1 Alpha ELISA Kit, 192 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Solid
Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
HIF-1 Alpha ELISA Kit, Human
Intra-assay CV <10%; inter-assay CV <10%
1 Kit(s)
≤30pg/mL
50μL
192 Assay(s)
Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
TMB
3.5 hr.
Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
450 to 550nm
Transcription factors
81.92 to 20,000pg/mL
Mouse and rat HIF-1A
Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Protein assays and analysis
Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
1 Kit(s)
Absorbance at 450nm minus absorbance at 550nm
For research use only. Not for use in diagnostic procedures
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
50μL
Human
Store kit at 2°-8°C, 2 to 8°C
192 Determinations
3.5 hr.
192
Human
Immunology
Documents