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Thermo Scientific™ HIF-1 alpha ELISA Kit, Human


Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Manufacturer: thermo scientific™  EHIF1A2

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Catalog No. ENEHIF1A2

  • / Each
 

Description & Specifications

Specifications

Assays 192
Measuring Range 450 to 550nm
Assay Range 81.92 to 20,000pg/mL
Protein Family Transcription Factors
Packaging Quantity 1 Kit(s)
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Sample Volume 50μL
Sensitivity ≤30pg/mL
Substrate Type TMB
Sample Size 50μL
Measurement Absorbance at 450nm minus absorbance at 550nm
Regulatory Status For research use only. Not for use in diagnostic procedures
Host Species Human
Sufficient For 192 Determinations
Storage Requirements 2 to 8°C
Size 192 Assay(s)
Test Time 3.5 hr.
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Research Discipline Immunology
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
For Use With (Application) Protein assays and analysis
Total Assay Time 3.5 hr.
Cross Reactivity Mouse and rat HIF-1A
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Detection Method Colorimetric
Precision Intra-assay CV <10%; inter-assay CV <10%
Product Type HIF-1 Alpha ELISA Kit, Human
Conjugate Streptavidin-HRP
Description HIF-1 Alpha ELISA Kit, 192 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Formulation Solid
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Quantity 1 Kit(s)
Quantity 192 assays
Species Human