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Thermo Scientific™ Heracell™ VIOS 250i Tri-Gas CO2 Incubators

Achieve your next breakthrough. This CO2 incubator delivers the performance reliability, ease of operation, and value required to support a range of culturing needs.


Capacity (English) Shelf 22/66 lb.
Capacity (Metric) 255 L
Capacity (Metric) Shelf 10/30 kg
Certifications/Compliance CE, CSA
Data Outputs USB, and optional 4-20mA
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Products 1
Catalog Number Mfr. No. Description CO2 Sensor Technology Material Features Price Quantity  
Catalog Number Mfr. No. Description CO2 Sensor Technology Material Features Price Quantity  
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Thermo Scientific™
Heracell VIOS 250i Tri-Gas C02 incubator single chamber IR Sensor Powder Coated Cold Rolled Steel N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Achieve your next breakthrough. The Thermo Scientific™ Heracell™ VIOS CO2  incubator is specifically designed to deliver the performance reliability, ease of operation, and value required to support a range of culturing needs from basic research to the demanding, leading-edge applications, so you're ready for whatever comes next.

Better solutions for optimal cell growth. Get added culturing flexibility with our CO2 incubators with variable oxygen control (tri-gas).

Direct Heat Chamber
  • 165L (5.8 cu ft) electropolished stainless steel chamber with compact footprint, easily stackable
  • Standard left hand door swing, with inner 3 segmented gas tight glass doory
THRIVE Active Airflow
  • In-chamber fan gently distributes clean, humidified air throughout the chamber ensuring homogeneous conditions
  • Fast recovery of all parameters ten minutes or less for stable culturing conditions
  • Auto-stop function disables fan operation during door openings to minimize air exchange
ISO 5 HEPA Filtration
  • In-chamber HEPA continuously filters the entire chamber air volume every 60 seconds
  • ISO 5 clean room air quality is achieved in the culture chamber within five minutes
  • Continuous protection by minimizing ability for contaminants to settle on interior surfaces
Protected Humidity Reservoir
  • Directly heated water reservoir provides stable high relative humidity levels, preventing culture desiccation
  • Cover limits particles and spilled media from settling into the reservoir
  • Condensation free inner chamber, prevents a breeding ground for contaminant
  • 5X faster humidity recovery than traditional water pan designs
  • For easier water handling, humidity reservoir may be filled or drained without the removal of shelves or cultures
In situ sensors and probes
  • Dual temperature probes for over temperature protection with operational back up
  • Choice of thermal conductivity (TC) with rh compensation or single beam, dual wavelength infrared (IR) CO2 sensor
  • O2 control ranges 1 to 21% (hypoxic) and 5 to 90% (hyperoxic)
Steri-Run, high temperature disinfection cycle
  • Overnight cycle simplifies cleaning and eliminates the need for separate autoclaving of parts
  • 180°C cycle assures uniform 6-log sterilization on all chamber surfaces eliminating biological contaminants
Intuitive, interactive iCAN™ Touchscreen Control
  • Convenient on-screen user prompts and reminders for easy navigation
  • Selectable languages simplify operation: English, Spanish, German, French, Italian, Japanese and Mandarin
  • Visibility to changes in culture environment on performance graphs and error and data logs
Ordering Information:

For 220V Tri-gas models, please order a Heracell VIOS single base model (51030287, 51030286, 51030478 or 51030476) with the factory installed O2 controlled option


22/66 lb.
10/30 kg
USB, and optional 4-20mA
629 mm
38.1 in.
968 mm
19.68 in.
215 lb.
3 std./12 max.
120 V
23.9 in.
774 mm
560 mm
50/60 Hz
934 mm
75 w during incubation, 125 w during sterlization
255 L
24.8 in.
Under 12 hours
26.4 in.
670 mm
500 mm
<93% at 37°C
Ambient +3°C to 55°C
30.5 in.
22.1 in.
607 mm
120 V, 50/60 Hz
36.8 in.
42mm access port, rear left