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Thermo Scientific™ Lab Vision™ HER-2/c-erbB-2/neu (Phospho-specific) Ab-18, Mouse Monoclonal Antibody


Provide accurate, reproducible results with the Thermo Scientific™ HER-2/c-erbB-2/neu (Phospho-specific) Ab-18, Mouse Monoclonal Antibody.

Manufacturer: thermo scientific™ lab vision™  MS1072PABX

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Catalog No. MS1072PABX

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Description & Specifications

Specifications

Cross Reactivity Human
Cross Reactivity Rat
Applications Immunofluorescence
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Isotype IgG1
Quantity 200µL
Regulatory Status RUO
Clone PN2A
Format Concentrated
Antigen HER-2/c-erbB-2/neu (Phospho-specific) Ab-18
Host Species Mouse
Immunogen Tyrosine-phosphorylated synthetic peptide corresponding to aa1242-1255 [Lys-TAENPE-pY-LGLDVPV] from the C-terminus of human c-erbB-2 protein (P-Tyr1248)
Research Discipline EGFR Family
Type Primary
Classification Monoclonal
Conjugate Unlabeled
Concentration 1mg/mL

Provide accurate, reproducible results with the Thermo Scientific™ HER-2/c-erbB-2/neu (Phospho-specific) Ab-18, Mouse Monoclonal Antibody.

c-erbB-2 (HER-2/neu) is a 185 kDa receptor tyrosine kinase belonging to the epidermal growth factor receptor family, which also includes EGFR (HER-1), c-erbB-3 (HER-3), and c-erbB-4 (HER-4). Amplification of the HER-2 gene with overexpression of the protein occurs in 20-30% of breast cancers, and tumor progression in mammary cancer is associated with elevated levels of tyrosine-phosphorylated neu and erbB-3. Tyr1248 is the phosphorylation site that is most tightly linked to oncogenic transformation and coupling to the ras/MAP kinase signaling pathway. Cases of invasive breast carcinoma with the phosphorylated receptor have displayed aggressive clinicopathological features and adverse prognoses. Activation of HER-2 is frequent in ductal carcinoma in situ suggesting that HER-2 signaling plays a critical role in the early stages of breast tumorigenesis.

Host Species: Mouse

Clone: PN2A

Isotype: IgG1

Species Reactivity: Human and Rat. Others not tested.

Epitope: aa 1242-1255 with pTyr

Immunogen: Tyrosine-phosphorylated synthetic peptide corresponding to aa 1242-1255 [Lys-TAENPE-pY-LGLDVPV] from the C-terminus of human HER-2 protein (P-Tyr1248)

Molecular Weight: 185 kDa

Positive Control: EGF-treated SKBR-3 cells. About 5% of breast CA are positive for phospho-HER-2/c-erbB-2/neu oncoprotein.

Cellular Localization: Membrane

Recommended for:

  • Immunohistochemistry
  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation (Not verified)
  • Flow Cytometry (Not verified)

Note:

USA: RUO; Int'l: RUO