GRP78, Alexa Fluor 488, clone: C38, eBioscience™
Mouse Monoclonal Antibody
Manufacturer: Life Technologies LS53976882
The protein encoded by this gene is a member of the heat shock protein 70 family. It is localized in the lumen of the endoplasmic reticulum , and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.Evidence also suggests that GRP78 plays a role in promoting tumor growth and metastasis by inhibiting apoptosis. GRP78 overexpression confers drug-resistance to chemotherapeutic agents and the level of expression can be correlated with pathologic grade and survival in breast, liver, prostate, colon and gastric cancers. Applications Reported: This C38 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This C38 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78’s involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintece of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer′s Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Glucose Regulated Protein, BiP, 78 kDa glucose-regulated protein, Immunoglobulin heavy chain-binding protein, Endoplasmic reticulum lumenal Ca(2+)-bin|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|Alexa Fluor® 488|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.