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Granzyme M Mouse anti-Human, eFluor® 660, Clone: 4B2G4, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS50977442

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Catalog No. 501124542



Description

Description

Description: Granzyme M is one of five granzyme serine proteases that have been indentified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Although not as well characterized as some of the other family members, Granzyme M has been found to exhibit some distinct functions. These include the non-cytotoxic inhibition of cytomegalovirus replication, induction of apoptosis in tumor cells, and contribution to the TLR4-induced inflammatory process. Applications Reported: This 4B2G4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4B2G4 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.
Specifications

Specifications

Granzyme M
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P51124
Granzyme M, 3421-, Met-1 serine protease, Hu-Met-1, Met-ase, Natural killer cell granular protease, GZMM, MET1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4B2G4
eFluor® 660
GZMM
Liquid
GZMM
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3004
Documents

For Research Use Only.