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Thermo Scientific™ Lab Vision™ γ Glutamylcysteine Synthetase (GCS)/Glutamate-cysteine Ligase Ab-1, Rabbit Polyclonal Antibody


Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments.

Manufacturer: thermo scientific™ lab vision™  RB1697PABX

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Catalog No. RB1697PABX

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Description & Specifications

Specifications

Antigen Gamma Glutamylcysteine Synthetase (GCS)/Glutamate-cysteine Ligase Ab-1
Applications Western Blotting
Classification Polyclonal
Concentration 1mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Non-Human Primate
Format Concentrated
Formulation Ab without BSA/Azide (Frozen)
Host Species Rabbit
Immunogen A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)
Isotype IgG
Quantity 1mL
Regulatory Status RUO
Research Discipline Enzyme
Type Primary

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments with Thermo Scientific™ γ Glutamylcysteine Synthetase (GCS)/Glutamate-cysteine Ligase Ab-1, Rabbit Polyclonal Antibody.

Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS)/Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Monkey, Mouse and Rat, Cow (weak). Others not known.

Epitope: aa 295-313

Immunogen: A synthetic peptide corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)

Molecular Weight: 73 kDa

Positive Control: Jurkat, MAD109, PC12 cells

Cellular Localization: Cytoplasmic

Recommended for:

  • Western Blotting
  • Immunoprecipitation (Denatured verified)

Note:

USA: RUO; Int'l: RUO