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Thermo Scientific™ Lab Vision™ γ Glutamylcysteine Synthetase (GCS)/Glutamate-cysteine Ligase Ab-1, Rabbit Polyclonal Antibody

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments.

Manufacturer:  Thermo Scientific™ Lab Vision™ RB1697P1ABX

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Catalog No. RB1697P1ABX


 

Specifications

Description

Ensure accurate, reproducible results in western blotting and immunoprecipitation experiments with Thermo Scientific™ γ Glutamylcysteine Synthetase (GCS)/Glutamate-cysteine Ligase Ab-1, Rabbit Polyclonal Antibody.

Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS)/Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Monkey, Mouse and Rat, Cow (weak). Others not known.

Epitope: aa 295-313

Immunogen: A synthetic peptide corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)

Molecular Weight: 73 kDa

Positive Control: Jurkat, MAD109, PC12 cells

Cellular Localization: Cytoplasmic

Recommended for:

  • Western Blotting
  • Immunoprecipitation (Denatured verified)

Note:

USA: RUO; Int'l: RUO

Description & Specifications

Specifications

Gamma Glutamylcysteine Synthetase (GCS)/Glutamate-cysteine Ligase Ab-1
Polyclonal
Unlabeled
Concentrated
Rabbit
IgG
RUO
Primary
Human,Murine,Non-human Primate
Western Blot
1mg/mL
Human,Mouse,Non-Human Primate
Ab without BSA/Azide (Frozen)
A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)
500μL
Enzyme
Polyclonal