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Gibco™ VEGF Recombinant Human Protein DFS Item


Recombinant Human Vascular Endothelial Cell Growth Factor (VEGF) is a bioactive protein intended for use in cell culture applications

Manufacturer: gibco™  PHC9393

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 Disclaimers

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Catalog No. PHC9393

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Description & Specifications

Specifications

For Use With (Application) Cell Culture; Mammalian Cell Culture; Cell Analysis; Cell Viability, Proliferation & Function; Stem Cell Research; Stem Cell Culture; Stem Cell Differentiation; Embryonic Stem Cell Culture; Hematopoietic Stem Cell Culture; Differentiation & Development
Format Lyophilized
Molecular Weight 40kDa
Purification Method Sequential Chromatography
Purity >95%
Quantity 1mg
Source Human
Storage Requirements Store this lyophilized preparation at 2 to 8°C. Upon reconstitution, apportion into working aliquots and store at ≤ –20°C. Avoid repeated freeze/thaw cycles.
Without Additives Carrier Protein
Structural Form Recombinant/Ligand
Protein Subtype VEGF (Vascular Endothelial Growth Factor)
Endotoxin Concentration <0.1ng/μg
Gene Alias VEGF
Assays Proliferation of HUVECs (Human Umbilical Vein Endothelial Cells)
Validated Application Cell Culture
Protein Family Growth Factors & Receptors
Expression System Mammalian
Protein Length 165 aa
Biological Activity ED50 range: 1-6ng/mL
Specific Reactivity 1.0 x 106 - 1.7 x 105 units/mg

VEGF exerts angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. VEGF has also been shown to be chemotactic for monocytes and osteoblasts.

  • High purity: no interference from other proteins or contaminants
  • High biological activity: more results with less protein
  • Proven compatibility: Gibco proteins bioassayed with Gibco media
  • High Purity Recombinant Bioactive Protein: to help ensure Gibco growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein
  • High Biological Activity: in-house activity testing of Gibco Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, anti-viral, and protease assays; specific activity is determined by the dose dependent proliferation of human umbilical vein cells (HUVEC)

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