Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.
Gentle on cells
Room temperature-stable for 24 months
Easy to use
Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts
Can be directly substituted for trypsin in existing protocols
Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS
Energy efficient, less hazardous, sustainable packaging