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Gibco™ RPMI 1640 Medium, powder DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer:  Gibco™ 31800089

 View more versions of this product

Catalog No. 31800089

Requires : Supplementation



  • RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer
  • Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
  • Store at 2°C to 8°C

Modified as follows:

  • With: L-glutamine, phenol red
  • Without: HEPES, sodium bicarbonate

Using RPMI

  • RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • In addition, the vitamins inositol and choline are present in very high concentrations
  • RPMI 1640 Medium contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • It uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation


RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Room Temperature
Phenol Red
10 L
L-Glutamine, Phenol Red
No Sodium Pyruvate
Animal Origin-Free
Standard Serum Supplementation
36 Months From Date of Manufacture
Leukemic cells
HEPES, Sodium Bicarbonate
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)


Product Certifications


Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.