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Gibco™ RPMI 1640 Medium, powder DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer:  Gibco™ 31800089

 View more versions of this product

Catalog No. 31800089



Requires : Supplementation

Description

Description

  • RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer
  • Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas
  • Store at 2°C to 8°C

Modified as follows:

  • With: L-glutamine, phenol red
  • Without: HEPES, sodium bicarbonate

Using RPMI

  • RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins
  • RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • In addition, the vitamins inositol and choline are present in very high concentrations
  • RPMI 1640 Medium contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • It uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
Specifications

Specifications

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Room Temperature
Powder
Phenol Red
10 L
L-Glutamine, Phenol Red
No Sodium Pyruvate
Animal Origin-Free
No HEPES
Sterile-filtered
Standard Serum Supplementation
Gibco™
L-Glutamine
36 Months From Date of Manufacture
Leukemic cells
HEPES, Sodium Bicarbonate
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.