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Gibco™ RPMI 1640 Medium, powder DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer:  Gibco™ LS31800089

 View more versions of this product

Catalog No. 31800089



Requires : Supplementation

Description

Description

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Modified as follows: with L-glutamine, phenol red; without HEPES, sodium bicarbonate
  • Contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation

Cell Culture, Mammalian Cell Culture

Specifications

Specifications

No HEPES
Room Temperature
Powder
Phenol Red
No Sodium Pyruvate
Standard Serum Supplementation
Gibco™
L-Glutamine
10L
SDS
Documents
Product Certifications

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.