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RPMI 1640 Medium, No Phenol Red DFS Item


Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer: life technologies  11835030

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Supplementation

Catalog No. 11835030

  • / Each
 

Description & Specifications

Specifications

Format Liquid
Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Para-Aminobenzoic Acid, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Quantity 500mL
Storage Requirements Storage: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives No Sodium Pyruvate; L-Glutamine; No Phenol Red

Roswell Park Memorial Institute (RPMI) 1640 Medium is suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Modified as follows: with L-glutamine; without HEPES, phenol red
  • Contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses a sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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