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Gibco™ RPMI 1640 Medium 
Originally developed to culture human leukemic cells in suspension and as a monolayer
Manufacturer: Gibco™ 11875168
Requires : Supplementation
Description
Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
- Modified as follows: with L-glutamine, phenol red; without HEPES
- Contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in high concentrations
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Specifications
| 1X | |
| Sterile-filtered | |
| Room Temperature | |
| Gibco™, SynerGy™ | |
| L-Glutamine | |
| 12 Months From Date of Manufacture | |
| leukemic cells | |
| HEPES | |
| HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas | |
| RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
| RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) | |
| Standard Serum Supplementation | |
| 5 L | |
| Liquid | |
| Phenol Red | |
| 5L | |
| Phenol Red, L-Glutamine | |
| No Sodium Pyruvate | |
| Animal Origin-Free | |
| No HEPES |
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.