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Gibco™ RPMI 1640 Medium DFS Item

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer:  Gibco™ 11875168

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Catalog No. 11875168



Requires : Supplementation

Description

Description

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

  • Modified as follows: with L-glutamine, phenol red; without HEPES
  • Contains reducing agent glutathione and high concentrations of vitamins
  • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
  • Vitamins inositol and choline are present in high concentrations
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Specifications

Specifications

1X
Sterile-filtered
Room Temperature
Gibco™, SynerGy™
L-Glutamine
12 Months From Date of Manufacture
leukemic cells
HEPES
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Standard Serum Supplementation
5 L
Liquid
Phenol Red
5L
Phenol Red, L-Glutamine
No Sodium Pyruvate
Animal Origin-Free
No HEPES
SDS
Documents
Product Certifications
Promotions

Promotions

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.