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Gibco™ RPMI 1640 Medium
Originally developed to culture human leukemic cells in suspension and as a monolayer
Manufacturer: Gibco™ 11875168
Requires : Supplementation
Description
Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
- Modified as follows: with L-glutamine, phenol red; without HEPES
- Contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in high concentrations
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Specifications
1X | |
Sterile-filtered | |
Room Temperature | |
Gibco™, SynerGy™ | |
L-Glutamine | |
12 Months From Date of Manufacture | |
leukemic cells | |
HEPES | |
HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas | |
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) | |
Standard Serum Supplementation | |
5 L | |
Liquid | |
Phenol Red | |
5L | |
Phenol Red, L-Glutamine | |
No Sodium Pyruvate | |
Animal Origin-Free | |
No HEPES |
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.