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Gibco™ RPMI 1640 Medium 
Originally developed to culture human leukemic cells in suspension and as a monolayer
Manufacturer: Gibco™ LS11875168
Requires : Supplementation
Description
Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
- Modified as follows: with L-glutamine, phenol red; without HEPES
- Contains reducing agent glutathione and high concentrations of vitamins
- Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
- Vitamins inositol and choline are present in high concentrations
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Gibco®, SynerGy™ | |
| Standard Serum Supplementation | |
| No Sodium Pyruvate | |
| Liquid | |
| Phenol Red |
| No HEPES | |
| 5L | |
| Room Temperature | |
| 5 L | |
| L-Glutamine |
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.