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Gibco™ MEM α, nucleosides, GlutaMAX™ Supplement DFS Item

Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells

Manufacturer:  Gibco™ 32571036

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Catalog No. 32571036

Requires : Supplementation



MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.

  • Gibco MEM α is modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid
  • Aailable without nucleosides for use as selection medium for DG44 and other DHFR negative cells
  • Gibco MEM α with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
  • Modified as follows: with ribonucleosides, deoxyribonucleosides, phenol red, GlutaMAX™
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture



Standard Serum Supplementation
500 ml
Phenol Red
Sustainable packaging
primary rat astrocytes
Low Glucose
HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
MEM α (Minimum Essential Medium α)
MEM α (Minimum Essential Medium α)
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Room Temperature
Gibco™, GlutaMAX™
12 Months From Date of Manufacture
Ribonucleosides, Deoxyribonucleosides, GlutaMAX I, Phenol Red
Sodium Pyruvate
Animal Origin-Free
Product Certifications


Product Intended Use: For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.