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MEM α, Nucleosides DFS Item


Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells

Manufacturer: life technologies  12571071

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Supplementation

Catalog No. 12571071

  • / Each of 10
 

Description & Specifications

Specifications

Format Liquid
Formulation 2'Deoxyguanosine, 2Deoxyadenosine, 2Deoxycytidine HCl, Adenosine, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cytidine, D-Calcium pantothenate, Folic Acid, Glycine, Guanosine, L-Alanine, L-Arginine, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Thymidine, Uridine, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Quantity 10 x 500mL
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. This MEM is modified as follows: ribonucleosides, deoxyribonucleosides, phenol red, L-glutamine.

  • Modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid
  • Available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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