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MEM α, No Nucleosides, Powder DFS Item

Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells

Manufacturer: life technologies  12000022

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Catalog No. 12000022


Description & Specifications


Format Powder
Formulation Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glycine, L-Alanine, L-Arginine, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Lipoic Acid, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, Vitamin B12, i-Inositol
Quantity 10 x 1L
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
With Additives Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. Life Technologies offers a variety of Gibco MEM α modifications for a range of cell culture applications. This MEM is modified as follows: with phenol red, L-glutamine; without ribonucleosides, deoxyribonucleosides, sodium bicarbonate.

  • MEM α can be used with variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells
  • Modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid
  • Available without nucleosides for use as selection medium for DG44 and other DHFR negative cells
  • Made with Earle's salts

Cell Culture, Mammalian Cell Culture

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