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MEM, NEAA, No Glutamine DFS Item

Minimum Essential Medium is commonly used of all cell culture media

Manufacturer: life technologies  10370088

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Catalog No. 10370088


Description & Specifications


Format Liquid
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, Folic Acid, Glycine, L-Alanine, L-Arginine, L-Asparagine-H2O, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
Packaging Sustainable packaging
Quantity 10 x 500mL
Storage Requirements Storage: 2-8°C. Protect from light. Shelf life : 12 months from date of manufacture
With Additives No Sodium Pyruvate; Low Glucose; No Glutamine; Phenol Red

MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification.

  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Modified as follows: with NEAA, low glucose and phenol red; without L-glutamine and HEPES
  • Made with Earle's salts
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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