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Medium 199 DFS Item

Originally developed for nutritional studies of chick embryo fibroblasts

Manufacturer: life technologies  11150059

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Catalog No. 11150059


Description & Specifications


Format Liquid
Formulation 2-deoxy-D-ribose, Adenine sulfate, Adenosine 5-phosphate, Adenosine 5-triphosphate, Alpha-tocopherol Phosphate, Ascorbic Acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Cholesterol, Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Guanine hydrochloride, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Menadione (Vitamin K3), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Vitamin A (acetate), Vitamin D2 (Calciferol), i-Inositol
Packaging Sustainable packaging
Quantity 500mL
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives No Sodium Pyruvate; Low Glucose; L-Glutamine; Phenol Red

Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. This M199 is modified as follows: with phenol red, L-glutamine; without HEPES.

  • Contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins
  • Available with Earle's salts for use in CO2 incubator, or with Hank's salts for use without CO2
  • Contains no proteins, lipids, or growth factors
  • Uses sodium bicarbonate buffer system (2.2g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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