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Gibco™ IMDM, powder DFS Item

Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells

Manufacturer:  Gibco™ LS12200036

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Catalog No. 12200036



Requires : Supplementation

Description

Description

IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate.

This IMDM is modified as follows: with L-glutamine, phenol red; without α-thioglycerol, 2-mercaptoethanol. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.

Cell Culture, Mammalian Cell Culture

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
HEPES
10 x 1L
Sodium Pyruvate
L-Glutamine
High Glucose
Gibco®
Room Temperature
Powder
Phenol Red
SDS
Documents

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.