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IMDM, Powder DFS Item

Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells

Manufacturer: life technologies  12200036

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Catalog No. 12200036


Description & Specifications


Format Powder
Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glycine, HEPES, L-Alanine, L-Arginine hydrochloride, L-Asparagine (freebase), L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Potassium Nitrate (KNO3), Pyridoxal hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Sodium Selenite (Na2SeO3-5H20), Thiamine hydrochloride, Vitamin B12, i-Inositol
Quantity 10 x 1L
Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
With Additives Sodium Pyruvate; High Glucose; L-Glutamine; Phenol Red

IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate.

This IMDM is modified as follows: with L-glutamine, phenol red; without α-thioglycerol, 2-mercaptoethanol. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.

Cell Culture, Mammalian Cell Culture

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