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IMDM, HEPES DFS Item


Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells

Manufacturer: life technologies  12440079

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Supplementation

Catalog No. 12440079

  • / Each of 6
 

Description & Specifications

Specifications

Format Liquid
Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glycine, HEPES, L-Alanine, L-Arginine hydrochloride, L-Asparagine (freebase), L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Potassium Nitrate (KNO3), Pyridoxal hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Sodium Selenite (Na2SeO3-5H20), Thiamine hydrochloride, Vitamin B12, i-Inositol
Packaging Sustainable packaging
Quantity 6 x 1000mL
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives Sodium Pyruvate; High Glucose; L-Glutamine; Phenol Red

  • It is modified as follows: with L-glutamine, phenol red; without a-thioglycerol, 2-mercaptoethanol
  • Includes selenium as well as additional amino acids and vitamins
  • Lacks iron, with potassium nitrate replacing ferric nitrate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.024g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture

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