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Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement Greener Choice DFS Item

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Manufacturer:  Gibco™ LS31765035

 View more versions of this product

Catalog No. 31765035

Requires : Supplementation



Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.

  • Contains zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
  • It is modified as follows: with GlutaMAX™, phenol red. Gibco F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
  • Contains no proteins or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture



500 ml
Phenol Red
Room Temperature
Gibco™, GlutaMAX™
Sustainable packaging
Sodium Pyruvate
Product Certifications

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.