missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement DFS Item

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Supplier:  Gibco™ 31765035

 View more versions of this product

Catalog No. 31765035

Requires : Supplementation



  • Designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells
  • F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells
  • This Ham's F-12 supplement is modified with GlutaMAX™ and phenol red indicator
  • Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations
  • It minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Gibco™ F-12 contains no proteins or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Store at 2°C to 8°C, protected from light


Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
Ham's F-12 Nutrient Mixture
Gibco™, GlutaMAX™
Room Temperature
CHO, COS-7, and rat prostate epithelial cells
Animal Origin-Free
12 Months From Date of Manufacture
GlutaMAX,Phenol Red,Sodium Pyruvate
Sustainable packaging
500 mL


Product Certifications


For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.