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Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement Greener Choice DFS Item

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Manufacturer:  Gibco™ LS31765092

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Catalog No. 31765092

Requires : Supplementation



Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.

  • Contains zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
  • It is modified as follows: with GlutaMAX™, phenol red. Gibco F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
  • Contains no proteins or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Sustainable packaging

Cell Culture, Mammalian Cell Culture



500 ml
Phenol Red
10 x 500mL
Room Temperature
Gibco™, GlutaMAX™
Sustainable packaging
Sodium Pyruvate

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.