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Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement DFS Item

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Manufacturer:  Gibco™ 31765092

 View more versions of this product

Catalog No. 31765092



Requires : Supplementation

Description

Description

  • Designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells
  • F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells
  • This Ham's F-12 supplement is modified with GlutaMAX™ and phenol red indicator
  • Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations
  • It minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format
  • Gibco™ F-12 contains no proteins or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH
  • Store at 2°C to 8°C, protected from light
Specifications

Specifications

1X
Sterile-filtered
Room Temperature
Liquid
Phenol Red
Sustainable packaging
Primary rat astrocytes
Sodium Pyruvate
Animal Origin-Free
No HEPES
Ham's F-12 Nutrient Mixture
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Gibco™, GlutaMAX™
GlutaMAX-I
12 Months From Date of Manufacture
10 x 500mL
GlutaMAX-I, Phenol Red
CHO, COS-7, and rat prostate epithelial cells
Ham's F-12 Nutrient Mixture
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.