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Grace's Insect Medium, Supplemented, Insect Hemolymph-Free, Lactalbumin Hydrolysate, Yeastolate DFS Item


Widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21

Manufacturer: life technologies  11605094

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Grace's Unsupplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.

Catalog No. 11605094

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Description & Specifications

Specifications

Cell Line Sf21, Sf9
Additive L-Glutamine
Format Liquid
Formulation Alpha-Ketoglutaric acid, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Fructose, D-Glucose (Dextrose), Folic Acid, Fumaric acid, Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt, L-Valine, Lactalbumin hydrolysate, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Malic acid, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Phosphate monobasic (Na2H2PO4-H2O), Succinic acid, Sucrose, Thiamine hydrochloride, Yeastolate, beta-Alanine, i-Inositol
Inorganic Salt Type Calcium, Magnesium
For Use With (Application) Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture
Quantity 500mL
Storage Requirements Storage conditions: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture

Originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS).

  • Contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate
  • Dual site cGMP manufacturing and quality system
  • Commonly supplemented with 10% Fetal Bovine Serum (FBS) before use

Cell Culture, Insect Cell Culture, Sf9 and Sf21 Cell Culture

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