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Freestyle™ CHO Expression Medium DFS Item


Animal origin-free, chemically defined, protein-free medium

Manufacturer: life technologies  12651014

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Catalog No. 12651014

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Description & Specifications

Specifications

Cell Line CHO, FreeStyle™ CHO-S Cells
Additive No glutamine, no phenol red
Format Liquid
For Use With (Application) Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Quantity 1000mL
Storage Requirements Storage conditions: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture

FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.

  • Formulation without hypoxanthine and thymidine for use with or without dhfr systems
  • Ability to support high-density suspension growth and transient transfection of CHO-S cells
  • Animal origin-free, protein-free and chemically defined formulation
  • Easy use as part of the FreeStyle MAX CHO Expression System for protein production
  • Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems

  • Gibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems
  • Non-dhfr systems require the addition of HT Supplement before use
Ability to support high-density suspension growth and transient transfection of CHO-S cells
  • Gibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptation
  • Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptation
  • Cells usually require adaptation from serum-containing formulations
  • Cells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagent
Animal origin-free, protein-free and chemically defined formulation
  • Gibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
  • Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition
Scalability in spinner flasks and bioreactors
  • Protein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactors
  • The appropriate spinner or impeller speed and seeding density should be optimized for each system
  • Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture