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Gibco™ DMEM, Powder, High Glucose, Pyruvate, No Sodium Bicarbonate Buffer DFS Item


Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, High Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications.

Manufacturer: gibco™  12800017

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Supplementation

Catalog No. 12800017

  • / Each of 10
 

Description & Specifications

Specifications

For Use With (Application) Mammalian cell culture
Format Powder
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, i-Inositol
Product Type DMEM media
Quantity 10 x 1L
Shelf Life 36 months from date of manufacture
Storage Requirements Storage: 2-8° C
With Additives High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate
Without Additives HEPES, Sodium Bicarbonate

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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