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Gibco™ DMEM Media, w/High Glucose, Pyruvate, No Glutamine DFS Item


Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM Media, w/High Glucose, Pyruvate, No Glutamine is used in human ex vivo tissue and cell culture processing applications.

Manufacturer: gibco™  LS10313039

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Supplementation

 Disclaimers

When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File.

Catalog No. 10313039

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Description & Specifications

Specifications

With Additives High Glucose, Phenol Red, Sodium Pyruvate
Storage Requirements Storage: 2°C to 8°C. Protect from light.
Certifications/Compliance cGMP - ISO 13485 standard
Product Type DMEM media
Without Additives L-glutamine, HEPES
Shelf Life 12 months from the date of manufacture
For Use With (Application) Mammalian cell culture
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, i-Inositol
Format Liquid
Quantity 10 x 500mL
Packaging Sustainable packaging

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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Accessories

12 months from the date of manufacture