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DMEM, High Glucose, HEPES DFS Item

Dulbecco's Modified Eagle Medium is a widely used basal medium for supporting growth of many different mammalian cells

Manufacturer: life technologies  12430112

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Catalog No. 12430112


Description & Specifications


Format Liquid
Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, HEPES, L-Arginine hydrochloride, L-Cystine 2HCl, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
Quantity 5L
Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
With Additives No Sodium Pyruvate; High Glucose; L-Glutamine; Phenol Red

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

  • Modified as follows: with high glucose, L-glutamine, HEPES, phenol red; without sodium pyruvate
  • Contains four times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

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