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Gibco™ DMEM, high glucose 
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.
Manufacturer: Gibco™ LS11965167
Requires : Supplementation
Description
- Contains four times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| High Glucose | |
| Gibco®, SynerGy™ | |
| Standard Serum Supplementation | |
| No Sodium Pyruvate | |
| Liquid | |
| Phenol Red |
| 4500 mg/L | |
| No HEPES | |
| 5L | |
| Room Temperature | |
| 5 L | |
| L-Glutamine | |
| Antibody and cell culture production, Mammalian cell culture |
When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.