Please login to your online account to display your discounted pricing

Gibco™ DMEM/F-12, HEPES, No Phenol Red DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12, HEPES, No Phenol Red is used in human ex vivo tissue and cell culture processing applications.

Manufacturer: gibco™  11039047

 View all options for this product

Catalog No. 11039047


Description & Specifications


For Use With (Application) Mammalian cell culture
Format Liquid
Formulation Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, HEPES, Hypoxanthine Na, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Product Type DMEM media
Quantity 10 x 500mL
Shelf Life 12 months from date of manufacture
Storage Requirements Storage: 2°C to 8°C. Protect from light.
With Additives Sodium Pyruvate; High Glucose; L-Glutamine
Without Additives Phenol red

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.