Please login to your online account to display your discounted pricing

Gibco™ Advanced DMEM DFS Item


Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEM is used in cell culture processing applications.

Manufacturer: gibco™  LS12491015

 View more versions of this product

Supplementation

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 12491015

  • / Each
 

Description & Specifications

Specifications

For Use With (Application) Mammalian cell culture
Shelf Life 12 months from date of manufacture
With Additives High Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red
Without Additives L-glutamine
Storage Requirements Storage: 2-8° C. Protect from light
Product Type DMEM media
Formulation AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric Sulfate, D-Calcium pantothenate, D-Glucose (Dextrose), Ethanolamine, Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glutathione (reduced), Glycine, Human Transferrin (Holo), Insulin Recombinant Full Chain, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Manganous Chloride, Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-H2O), Sodium Pyruvate, Sodium Selenite, Thiamine hydrochloride, i-Inositol
Quantity 500mL
Format Liquid
Packaging Sustainable packaging

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

Accessories

12 months from date of manufacture