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Gibco™ Advanced DMEM/F-12 DFS Item

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEMF/12 is used in cell culture processing applications.

Manufacturer: gibco™  12634010

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Catalog No. 12634010


Description & Specifications


For Use With (Application) Mammalian cell culture
Format Liquid
Formulation AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ethanolamine, Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glutathione monosodium, Glycine, Human Transferrin (Holo), Hypoxanthine Na, Insulin Recombinant Full Chain, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Manganous Chloride, Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Reducing Agents, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Sodium Selenite, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Packaging Sustainable packaging
Product Type DMEM/F-12 media
Quantity 500mL
Shelf Life 12 months from date of manufacture
Storage Requirements Storage: 2-8° C. Protect from light
With Additives Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red
Without Additives L-glutamine, HEPES

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12