Please login to your online account to display your discounted pricing

Thermo Scientific™ GeneJET RNA Purification Kit


Silica membrane-based total RNA purification from mammalian blood, cell cultures, and tissues as well as insect, yeast, and bacteria. Provided in a convenient spin-column format.

Manufacturer: thermo scientific™  K0731

 View more versions of this product

Catalog No. FERK0731

  • / Each
 

Description & Specifications

Specifications

Description GeneJET™ RNA Purification Kit
For Use With (Application) Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assay
Includes Proteinase K, Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET RNA Purificatio, Columns (pre-assembled with Collection Tubes), Collection Tubes (2mL), Collection Tubes (1.5mL), Detailed Protocol
No. of Reactions 50 Preps

GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

  • Universal– can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
  • Efficient– high yields of total RNA.
  • Fast– 15 minutes after lysis step
  • Pure– an A260/280ratio > 1.9
  • Convenient– spin columns are capped and assembled with collection tubes

Applications

  • Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
    • RT-PCR
    • RT-qPCR
    • Northern blotting
    • Nuclease protection assay
  • Proteinase K
  • Lysis Buffer
  • Wash Buffer 1 (concentrated)
  • Wash Buffer 2 (concentrated)
  • Water, nuclease-free
  • GeneJETRNA Purification Columns (pre-assembled with Collection Tubes)
  • CollectionTubes(2ml)
  • Collection Tubes (1.5ml)
  • Detailed Protocol