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GE Healthcare Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

$218.15 - $794.84

Specifications

Bed Volume 19 to 230L
Diameter (Metric) Inner 40 to 140cm
Product Type Sephacryl S-500 HR media
Flow Rate >125cm/hr.
Format Solid Spherical Bead
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-002-054

 
ge healthcare
17061301
750mL Each for $794.84

45-002-053

 
ge healthcare
17061310
150mL Each for $218.15
Description & Specifications

Specifications

Bed Volume 19 to 230L
Diameter (Metric) Inner 40 to 140cm
Product Type Sephacryl S-500 HR media
Flow Rate >125cm/hr.
Format Solid Spherical Bead
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
Height (English) 5.9 in.
Height (Metric) 15cm
Max. Pressure 100kPa
Particle Size 25 to 75μm
pH Range 3 to 11
Sample Type Polysaccharides, macromolecules and small plasmids
Storage Requirements 4° to 30°C
Support Type Allyl Dextran and N, N'-methylenebisacrylamide

Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

  • For separating polysaccharides, macromolecules with extended structures, and small plasmids
  • Excellent purification over a wide molecular weight range
  • High reproducibility due to long-term chemical and physical stability
  • High flow rates and recoveries
  • Well-suited to industrial-scale use
  • Storage conditions: 20% ethanol; 4° to 30°C
  • Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

  • 1M acetic acid, 8M urea
  • 6M guanidine-HCl, 1% SDS
  • 2M NaCl, 24% ethanol
  • 30% propanol, 30% acetonitrile
  • 0.5M NaOH (for cleaning)
pH Stability
  • 3 to 11 (long-term and working)
  • 2 to 13 (short-term)
Physical Stability:
  • Negligible volume variation due to changes in pH or ionic strength
  • Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
  • Antimicrobial agent: 20% ethanol