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Cytiva rProtein A Sepharose™ Fast Flow Affinity Media
Designed for purification of monoclonal and polyclonal antibodies at labs and process scales
Supplier: Cytiva 17127901
Description
- Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
- High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
- No mammalian components involved during manufacturing process
- Matrix: 4% cross-linked agarose
- Average particle size: 90μm (d50V)
- Ligand: 6mg rProtein A/mL medium (E. coli)
- Binding capacity: â½50mg human IgG/mL media
- pH stability working range: 3 to 10
- Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed
Specifications
| 5 mL | |
| Stable in aqueous Buffers commonly used in Protein A Chromatography | |
| 50 mg Human lgG/mL medium2 | |
| 150 to 250 cm/Hoμr | |
| 9.84 in. | |
| 100 kPa | |
| 2 to 9 | |
| Recombinant Protein A (E. coli) |
| For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales | |
| 2°C to 8°C | |
| rProtein A Sepharose Fast Flow Media | |
| 60 to 165 μm | |
| 25 cm | |
| 4% Cross-Linked Agarose | |
| 20% Ethanol |