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GE Healthcare Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

$462.52 - $7,105.20

Specifications

Product Type Ni Sepharose excel chromato graphy media
Flow Rate 150 to 600cm/hr.
Particle Size 90μm
pH Range 2 to 12
Storage Requirements 4° to 30°C
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-003-011

 
ge healthcare
17371202
100mL Each for $1,779.40

45-003-012

 
ge healthcare
17371203
500mL Each for $7,105.20

45-002-820

 
ge healthcare
17371201
25mL Each for $462.52
Description & Specifications

Specifications

Product Type Ni Sepharose excel chromato graphy media
Flow Rate 150 to 600cm/hr.
Particle Size 90μm
pH Range 2 to 12
Storage Requirements 4° to 30°C
Support Type 6% Highly Cross-linked Agarose

Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures.

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity.
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling.
  • Choose between several formats for screening and preparative purification of histidine-tagged proteins.
  • Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions.
  • Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.