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GE Healthcare Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

$91.43 - $5,382.00

Specifications

Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Product Type Ni Sepharose 6 FF chromato graphy media
Flow Rate 600cm/hr.
Height (English) 9.84 in.
Height (Metric) 250mm
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Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
45-002-985 GE Healthcare
17531802
100mL Each for $1,252.00
45-002-984 GE Healthcare
17531801
25mL Each for $374.00
45-002-987 GE Healthcare
17531806
5mL Each for $91.43
45-002-986 GE Healthcare
17531803
500mL Each for $5,382.00
Specifications

Description

Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Description & Specifications

Specifications

0.1M HCl, 0.1M NaOH, 8M Urea
600cm/hr.
250mm
90μm
4° to 30°C
Ni Sepharose 6 FF chromato graphy media
9.84 in.
1 bar
3 to 12
6% Cross-linked Agarose