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GE Healthcare IMAC Sepharose™ High Performance Media

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

$1,302.00 - $1,302.00

Catalog Number Mfr. No. Price Quantity    


ge healthcare
Each for $1,302.00
Description & Specifications

  • 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.