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Cytiva TempliPhi™ Large Construct Kit
Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA specifically from large constructs (e.g., fosmids and BACs) sufficient for DNA sequencing
Supplier: Cytiva 25640080
Includes: Sample buffer, Reaction buffer, and Enzyme mix
Description
- Start from microliter quantities of purified templates, as well as bacterial culture or glycerol stocks
- Generate 5μg amounts of template DNA for use directly in cycle sequencing reactions without purification
- Simplified process, 20 minutes hands-on time
- Amplification is completed in 18 hours with no need for thermal cycling
Specifications
| Store the kit at -70°C.The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. | |
| Sequencing Template Amplification Kits and Reagents | |
| 1000 | |
| 18 h |
| illustra TempliPhi Large Construct Kit uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates specifically from large DNA vectors (e.g., fosmids and BACs). | |
| 9mL Sample Buffer, 9mL Reaction Buffer, 500μL Enzyme mix | |
| illustra TempliPhi Large Construct Kit |