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GE Healthcare illustra™ TempliPhi™ 100/500 Amplification Kit

Use amplified DNA directly for cycle sequencing without purification. GE Healthcare illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.

Manufacturer:  GE Healthcare 25640050

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Catalog No. 45-002-265



Description

Description

  • Generate microgram quantities of template DNA from picogram amounts of starting material
  • Completed reactions contain uniform quantities of DNA irrespective of input amount, allowing for use directly in sequencing reactions without quantification
  • Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA without the need for purification
  • Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation
  • Contain separate, unmixed components for enhanced stability

Specifications

Specifications

illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA
illustra TempliPhi 500 Amplification Kit
The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°C Thaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification
Sequencing Template Amplification Kits and Reagents
500
500 Reactions
4-18 h depending on starting material
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