The purification process involves minimal shearing with typical yields of 4 to 6 μg of genomic DNA per 200μL sample with volumes accommodated up to 1mL. The method uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a novel silica-membrane. The elution volume can be modified as necessary for most downstream molecular biological applications.
- High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
- Demonstrated application to various types of whole blood including human, horse, rabbit, rat and mouse
- Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
- Validated in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
|Isolation of high-quality genomic DNA from whole Blood, buffy coat, bone marrow and nucleated red Blood cells|
|Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card|