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GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

$55.11 - $3,505.00

Specifications

Particle Size 60 to 160μm
Product Type HiTrap Talon™ crude prepacked Column
Flow Rate 1 to 4mL/min.
For Use With (Application) For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Ligand Type Talon™ Superflow Cobalt-IMAC
View More Specs


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
45-002-386 GE Healthcare
28953767
5 x 5mL Each for $769.00
45-002-385 GE Healthcare
28953766
5 x 1mL Each for $213.00
45-000-502 GE Healthcare
29048565
1 x 1mL Each for $55.11
45-002-387 Ge Healthcare Biosciences
28953805
100 x 1mL Each for $3,505.00
45-002-388 Ge Healthcare Biosciences
28953809
100 x 5mL N/A N/A  
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Specifications

Description

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.

Description & Specifications

Specifications

60 to 160μm
1 to 4mL/min.
Talon™ Superflow Cobalt-IMAC
6% Cross-Linked Agarose
3 to 12
4 to 8°C
HiTrap Talon™ crude prepacked Column
For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Polypropylene
3 bar
20% Ethanol
Up to 20mg Histidine-tagged Protein2