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GE Healthcare HiTrap™ TALON™ Crude Prepacked Columns

For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).   GE Healthcare Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.

$54.56 - $3,469.52

Specifications

Particle Size 60 to 160μm
Product Type HiTrap Talon™ crude prepacked Column
Flow Rate 1 to 4mL/min.
For Use With (Application) For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Ligand Type Talon™ Superflow Cobalt-IMAC
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-002-386

 
ge healthcare
28953767
5 x 5mL Each for $761.36

45-002-385

 
ge healthcare
28953766
5 x 1mL Each for $210.80

45-000-502

 
ge healthcare
29048565
1 x 1mL Each for $54.56

45-002-387

 
ge healthcare biosciences
28953805
100 x 1mL Each for $3,469.52

45-002-388

 
ge healthcare biosciences
28953809
100 x 5mL CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description & Specifications

Specifications

Particle Size 60 to 160μm
Product Type HiTrap Talon™ crude prepacked Column
Flow Rate 1 to 4mL/min.
For Use With (Application) For purifiCatIon of Histidine-tagged recombinant Proteins by immobilized Metal affinity chromatography, used Buffers and easily regenerated with 0.5 M NaOH
Ligand Type Talon™ Superflow Cobalt-IMAC
Material Polypropylene
Matrix 6% Cross-Linked Agarose
Max. Pressure 3 bar
pH Range 3 to 12
Storage Buffer 20% Ethanol
Storage Requirements 4 to 8°C
Binding Capacity Up to 20mg Histidine-tagged Protein1

Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

  • Prepacked with cobalt-based chromatography media
  • Binding capacity approximately 7mg MBP
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
  • 1 and 5mL columns

Media
  • Ligand: TALON™ Superflow Cobalt-IMAC
  • Matrix: 6% cross-linked agarose
  • Average particle size: 60 to 160μm
  • pH stability working range: 3 to 12

1mL columns

  • Column I.D.: 7mm
  • Flow velocity: 1 to 4mL/min.

5mL columns
  • Column I.D.: 16
  • Flow velocity: 5 to 20mL/min.