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Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
$80.92 - $1130.46
Specifications
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
|---|---|
| Storage Requirements | 4°C to 8°C |
| Product Type | HiTrap Talon crude prepacked Column |
| Flow Rate | 1 to 4 mL/min |
| Particle Size | 60 to 160 μm |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
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45-002-386
|
Cytiva
28953767 |
5 x 5 mL |
Each for $1,130.46
|
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45-002-385
|
Cytiva
28953766 |
5 x 1 mL |
Each for $313.51
|
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|
45-000-502
|
Cytiva
29048565 |
1 x 1 mL |
Each for $80.92
|
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Description
Cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Allows protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
- Prepacked with cobalt-based chromatography media
- Binding capacity approximately 7mg MBP
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
- 1 and 5mL columns
Media
- Ligand: TALON™ Superflow Cobalt-IMAC
- Matrix: 6% cross-linked agarose
- Average particle size: 60 to 160μm
- pH stability working range: 3 to 12
1mL columns
- Column I.D.: 7mm
- Flow velocity: 1 to 4mL/min.
5mL columns
- Column I.D.: 16
- Flow velocity: 5 to 20mL/min.
Specifications
| For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH | |
| HiTrap Talon crude prepacked Column | |
| 60 to 160 μm | |
| 3 bar | |
| 3 to 12 | |
| Talon Superflow Cobalt-IMAC |
| 4°C to 8°C | |
| 1 to 4 mL/min | |
| Polypropylene | |
| 6% Cross-Linked Agarose | |
| 20% Ethanol |