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GE Healthcare HiTrap™ rProtein A FF Columns

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. GE Healthcare Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

$390.60 - $3,228.96

Specifications

Binding Capacity ≈50mg Human IgG/mL
For Use With (Application) For preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, Serum and Ascites
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Storage Requirements 4° to 30°C
View More Specs
Products
Catalog Number Mfr. No. Flow Rate Quantity Price Quantity    

45-000-262

 
ge healthcare
17-5079-01
<4mL/min. 5 x 1mL Pack of 5 Each for $806.00

45-000-263

 
ge healthcare
17-5079-02
<4mL/min. 2 x 1mL Pack of 2 Each for $390.60

45-000-264

 
ge healthcare
17-5080-01
<20mL/min. 1 x 5mL Each for $720.44

45-000-265

 
ge healthcare
17-5080-02
<20mL/min. 5 x 5mL Pack of 5 Each for $3,228.96
Description & Specifications

Specifications

Binding Capacity ≈50mg Human IgG/mL
For Use With (Application) For preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, Serum and Ascites
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Storage Requirements 4° to 30°C

  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
  • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
  • Average particle size: 90μm
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Available as 1mL and 5mL columns
Media
  • Ligand: Recombinant protein A (E.coli)
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: ∽50mg human IgG/mL
  • pH stability working range: 3 to 10

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.