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GE Healthcare HiTrap™ IMAC Columns

For optimizing purification of histidine-tagged proteins when Ni +2 is not the best choice of metal ion

$184.76 - $753.92

Specifications

Flow Rate <4mL/min.
For Use With (Application) For optimizing purification of His-tagged Proteins
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system
Material Polypropylene
Max. Pressure 42psi
View More Specs
Includes:  Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems
Products
Catalog Number Mfr. No. Diameter (English) Inner Particle Size Quantity Price Quantity    

45-000-163

 
ge healthcare
17-0920-03
0.27 in. 34μm 5 x 1mL Pack of 5 Each for $221.96

45-000-164

 
ge healthcare
17-0920-05
0.62 in. 34μm 5 x 5mL Pack of 5 Each for $753.92

45-000-167

 
ge healthcare
17092102
0.27 in. 90μm 5 x 1mL Pack of 5 Each for $184.76

45-000-168

 
ge healthcare
17-0921-04
0.62 in. 90μm 5 x 5mL Pack of 5 Each for $638.60
Description & Specifications

Specifications

Flow Rate <4mL/min.
For Use With (Application) For optimizing purification of His-tagged Proteins
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system
Material Polypropylene
Max. Pressure 42psi
pH Range 3 to 12
Storage Requirements 4° to 30°C

  • Conveniently charge with your metal of choice
  • Can be used with a syringe, peristaltic pump, or liquid chromatography system
  • Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
  • High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
  • Stable in the presence of all commonly used buffers
  • Available in 1mL and 5mL sizes