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Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
$287.13 - $1116.70
Specifications
| Buffer | 6M Guanidine Hydrochloride, 8M Urea |
|---|---|
| Product Type | HiTrap chelating HP column |
| Height (English) | 0.98 in. |
| Height (Metric) | 25mm |
| Ligand Type | Iminodiacetic Acid |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity | |||||
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45-000-061
|
Cytiva
17040901 |
1 x 5mL |
Each for $287.13
|
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45-000-060
|
Cytiva
17040801 |
5 x 1mL |
Pack of 5 Each for $316.85
|
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45-000-062
|
Cytiva
17040903 |
5 x 5mL |
Pack of 5 Each for $1,116.70
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Description
- Uses different kinds of metal ions
- Binding capacity ∽12mg histidine-tagged protein/mL medium
- Metal ion capacity ∽23μmol Cu2+/mL medium
- Prepacked with Chelating Sepharose High Performance media
- Iminoacetic acid coupled to a 6% agarose matrix by ether linkage
Specifications
| 6M Guanidine Hydrochloride, 8M Urea | |
| 0.98 in. | |
| Iminodiacetic Acid | |
| 3 to 13 | |
| 6% Highly Cross-linked Agarose | |
| Affinity Column |
| HiTrap chelating HP column | |
| 25mm | |
| 3 bar | |
| 34μm | |
| 4° to 30°C | |
| IMAC and subsequent purification of Polyhistidine tagged Proteins |