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GE Healthcare HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

$195.92 - $761.36

Specifications

Buffer 6M Guanidine Hydrochloride, 8M Urea
Product Type HiTrap chelating HP column
Flow Rate <20mL/min.
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Height (English) 0.98 in.
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-000-062

 
ge healthcare
17-0409-03
5 x 5mL Pack of 5 Each for $761.36

45-000-061

 
ge healthcare
17-0409-01
1 x 5mL Each for $195.92

45-000-060

 
ge healthcare
17-0408-01
5 x 1mL Pack of 5 Each for $215.76
Description & Specifications

Specifications

Buffer 6M Guanidine Hydrochloride, 8M Urea
Product Type HiTrap chelating HP column
Flow Rate <20mL/min.
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Height (English) 0.98 in.
Height (Metric) 25mm
Ligand Type Iminodiacetic Acid
Max. Pressure 3 bar
Particle Size 34μm
pH Range 3 to 13
Storage Requirements 4° to 30°C
Support Type 6% Highly Cross-linked Agarose

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage