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GE Healthcare HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

$197.88 - $769.00

Specifications

Buffer 6M Guanidine Hydrochloride, 8M Urea
Product Type HiTrap chelating HP column
Flow Rate <4mL/min.
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Height (English) 0.98 in.
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Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
45-000-062 GE Healthcare
17040903
5 x 5mL Pack of 5 Each for $769.00
45-000-061 GE Healthcare
17040901
1 x 5mL Each for $197.88
45-000-060 GE Healthcare
17040801
5 x 1mL Pack of 5 Each for $218.00
45-002-910 GE Healthcare
17040905
100 x 5mL N/A N/A  
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Specifications

Description

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

Description & Specifications

Specifications

6M Guanidine Hydrochloride, 8M Urea
<4mL/min.
0.98 in.
Iminodiacetic Acid
34μm
4° to 30°C
HiTrap chelating HP column
IMAC and subsequent purification of Polyhistidine tagged Proteins
25mm
3 bar
3 to 13
6% Highly Cross-linked Agarose