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GE Healthcare HisTrap™ FF Columns: 16 × 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). GE Healthcare Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

$190.96 - $190.96

Specifications

Bed Volume 1mL
Support Type 6% Cross-linked Agarose
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Products
Catalog Number Mfr. No. Quantity Price Quantity    

45-000-336

 
ge healthcare
17-5319-01
5 × 1mL Pack of 5 Each for $190.96
Description & Specifications

Specifications

Bed Volume 1mL
Support Type 6% Cross-linked Agarose

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12