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GE Healthcare HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.   GE Healthcare Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

$72.64 - $912.00


Product Type HisTrap Excel Column
Material Polypropylene
pH Range 2 to 12
Pressure 5 bar
Storage Requirements 4° to 30°C
View More Specs

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Catalog Number Mfr. No. Diameter (Metric) Inner Flow Rate Quantity Price Quantity  
Catalog Number Mfr. No. Diameter (Metric) Inner Flow Rate Quantity Price Quantity  
45-003-013 GE Healthcare
7mm 150 to 600cm/hr. 5 x 1mL Each for $278.00
45-003-014 GE Healthcare
16mm 5 to 20mL/min. 5 x 5mL Each for $912.00
45-000-505 GE Healthcare
7mm 1 to 4mL/min. 1mL Each for $72.64


The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.

Description & Specifications


HisTrap Excel Column
2 to 12
4° to 30°C
5 bar
6% Highly Cross-linked Agarose