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GE Healthcare His SpinTrap™ Spin Columns and Kit

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. GE Healthcare Life Sciences™ His SpinTrap™ Spin Columns and Kit include prepacked, single-use spin columns.

$477.40 - $481.12

Specifications

Includes 1 Pack His SpinTrap and 1 pack His Buffer kit
Storage Requirements 4° to 30°C
Support Type 6% Highly Cross-linked Agarose
View More Specs
Products
Catalog Number Mfr. No. Product Type Price Quantity    

45-001-427

 
ge healthcare
28-4013-53
His SpinTrap Spin Column Pack of 50 Each for $477.40

45-002-017

 
ge healthcare
28-9321-71
His SpinTrap Kit Each for $481.12
Description & Specifications

Specifications

Includes 1 Pack His SpinTrap and 1 pack His Buffer kit
Storage Requirements 4° to 30°C
Support Type 6% Highly Cross-linked Agarose

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750μg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34μm

Kit contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)