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GE Healthcare HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccarides, macromolecules with extended structures and small plasmids

$806.00 - $1,077.56

Specifications

For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromato graphy systems
Height (English) 23.62 in.
Height (Metric) 60cm
Material Polypropylene
View More Specs
Products
Catalog Number Mfr. No. Bed Volume Diameter (Metric) Inner Flow Rate Product Type Price Quantity    

45-002-992

 
ge healthcare
28935606
120mL 1.6cm <0.5mL/min. HiPrep Sephacryl S-500 HR column Each for $806.00

45-002-993

 
ge healthcare
28935607
320mL 2.6cm <1.3mL/min. HiPrep Sephacryl S-500 HR column Each for $1,077.56
Description & Specifications

Specifications

For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromato graphy systems
Height (English) 23.62 in.
Height (Metric) 60cm
Material Polypropylene
Max. Pressure 22psi
Particle Size 25 to 75μm
pH Range 3 to 11
Quantity Each
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Storage Requirements 4° to 30°C
Support Type Allyl Dextran and N, N'-methylenebisacrylamide

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107