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GE Healthcare HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

For reproducible protein purification, separating polysaccarides, macromolecules with extended structures and small plasmids

$856.43 - $1,139.85

Specifications

For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromato graphy systems
Height (English) 23.62 in.
Height (Metric) 60cm
Material Polypropylene
View More Specs

Products 3
Catalog Number Mfr. No. Bed Volume Diameter (Metric) Inner Product Type Flow Rate Price Quantity  
Catalog Number Mfr. No. Bed Volume Diameter (Metric) Inner Product Type Flow Rate Price Quantity  
45-002-992 View Documents GE Healthcare
28935606
120 mL 1.6cm HiPrep 16/60 Sephacryl S-500 HR < 1.3 mL/min Each for $856.43
 
45-002-993 View Documents GE Healthcare
28935607
320 mL 2.6cm HiPrep 26/60 Sephacryl S-500 HR < 0.5 mL/min Each for $1,139.85
 
45-002-990 View Documents GE Healthcare
28935604
120 mL 1.6cm HiPrep 16/60 Sephacryl S-400 HR < 0.5 mL/min Each for $856.43
 
Description

Description

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

Specifications

Specifications

Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
23.62 in.
Polypropylene
25 to 75μm
Each
4 to 30°C, 20% Ethanol
Low to medium pressure AKTA™ systems or pumped chromato graphy systems
60cm
1.5 bar [0.15 MPa] (22 psi)
3 to 11
Polysaccharides, macromolecules with extended structures and even small plasmids
Allyl Dextran and N, N'-methylenebisacrylamide