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GE Healthcare Dharmacon Accell™ Control siRNA Kits


Accell siRNA represents a breakthrough technology to empower RNAi, without transfection reagents, viral vectors, or instrumentation

Manufacturer: ge healthcare dharmacon  K005000G102

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Catalog No. K005000G102

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Description & Specifications

Specifications

Color Green
For Use With (Application) Assessing Accell™ technology in a given cell type and to ensure effective controls during initial gene silencing experiments with Accell™ siRNA reagents
Includes 5nM Tubes of each of the four validated control siRNAs (Accell™ non-targeting siRNA #1, Accell™ Cyclophilin B siRNA and GAPD siRNA, Accell™ green non-targeting siRNA), 5X siRNA Buffer, 100mL delivery m
Product Type Accell siRNA Control Kit (Green)
Quantity Each
Species Mouse

The control kits are designed to be a starting point for assessing Accell technology in a given cell type, and to ensure effective controls during initial gene silencing experiments with Accell siRNA reagents.

All kits contain:

  • Accell Nontargeting siRNA #1: Distinguish general cellular responses to delivery methods and potential off-target effects from siRNA-mediated sequence-specific silencing
  • Accell Cyclophilin B siRNA and GAPD siRNA: Species-specific siRNAs to provide a positive control for silencing and validation of experimental design and detection methodologies
  • 5X siRNA buffer: The recommended buffer for resuspension of Accell siRNA
  • Accell siRNA delivery media: Specially formulated and optimized for Accell siRNA delivery; required for use with Accell siRNA reagents

Accell siRNA Control Kits (Green) also contain:

  • Accell Green Nontargeting siRNA: FAM-labeled Accell siRNA provides highly reliable qualitative assessment of uptake as determined by fluorescent microscopy to evaluate cytoplasmic localization of the dye-labeled Accell siRNA
    • Filter: FITC
    • Absorbance: 494nm
    • Emission Max: 520nm
Accell siRNA Control Kits (Red) also contain:
  • Accell Red Nontargeting siRNA: Dy-547-labeled Accell siRNA provides highly reliable qualitative assessment of uptake as determined by fluorescent microscopy to evaluate cytoplasmic localization of the dye-labeled Accell siRNA
    • Filter: Cy™3, Rhodamine, or PE
    • Absorbance: 557nm
    • Emission Max: 570nm